pgadt7 rec vectors Search Results


pgadt7 rec vector  (TaKaRa)
99
TaKaRa pgadt7 rec vector
Pgadt7 Rec Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgadt7 rec vector/product/TaKaRa
Average 99 stars, based on 1 article reviews
pgadt7 rec vector - by Bioz Stars, 2026-03
99/100 stars
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pgadt7 rec vector  (New England Biolabs)
99
New England Biolabs pgadt7 rec vector
CbABF1 subcellular localization and transactivation activity assays. (A) Localization of p35S:CbABF1-YFP in tobacco leaves, DAPI staining was used to indicate the nuclei. Bars = 15 μm. (B) A schematic diagram of the CbABF1 protein sequence and its truncated fragments that were fused to the GAL4 DNA-binding domain expressed from the yeast vector pGBKT7. The bZIP domain and four conserved domains are indicated. (C) Transactivation analysis of CbABF1 in yeast. The constructs for expression of the GAL4 DNA-binding domain fused with CbABF1, CbABF1Δ1, and CbABF1Δ2 were co-transformed with empty <t>pGADT7</t> into yeast strain AH109, respectively. A 10-fold dilution series (10 -1 to 10 -3 ) of transformants was spotted on SD medium without Leu and Trp (–LW), or without Leu, Trp, and His (–LWH) plus 35 mM 3-amino-1,2,4-triazole (3-AT). The plates were incubated at 30°C for 4 days and subjected to the X-gal assay. Empty pGBKT7 with empty pGADT7 was used as the negative control.
Pgadt7 Rec Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgadt7 rec vector/product/New England Biolabs
Average 99 stars, based on 1 article reviews
pgadt7 rec vector - by Bioz Stars, 2026-03
99/100 stars
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pgadt7 rec tamyb44 d  (TaKaRa)
86
TaKaRa pgadt7 rec tamyb44 d
CbABF1 subcellular localization and transactivation activity assays. (A) Localization of p35S:CbABF1-YFP in tobacco leaves, DAPI staining was used to indicate the nuclei. Bars = 15 μm. (B) A schematic diagram of the CbABF1 protein sequence and its truncated fragments that were fused to the GAL4 DNA-binding domain expressed from the yeast vector pGBKT7. The bZIP domain and four conserved domains are indicated. (C) Transactivation analysis of CbABF1 in yeast. The constructs for expression of the GAL4 DNA-binding domain fused with CbABF1, CbABF1Δ1, and CbABF1Δ2 were co-transformed with empty <t>pGADT7</t> into yeast strain AH109, respectively. A 10-fold dilution series (10 -1 to 10 -3 ) of transformants was spotted on SD medium without Leu and Trp (–LW), or without Leu, Trp, and His (–LWH) plus 35 mM 3-amino-1,2,4-triazole (3-AT). The plates were incubated at 30°C for 4 days and subjected to the X-gal assay. Empty pGBKT7 with empty pGADT7 was used as the negative control.
Pgadt7 Rec Tamyb44 D, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgadt7 rec tamyb44 d/product/TaKaRa
Average 86 stars, based on 1 article reviews
pgadt7 rec tamyb44 d - by Bioz Stars, 2026-03
86/100 stars
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pgadt7 rec  (TaKaRa)
86
TaKaRa pgadt7 rec
CbABF1 subcellular localization and transactivation activity assays. (A) Localization of p35S:CbABF1-YFP in tobacco leaves, DAPI staining was used to indicate the nuclei. Bars = 15 μm. (B) A schematic diagram of the CbABF1 protein sequence and its truncated fragments that were fused to the GAL4 DNA-binding domain expressed from the yeast vector pGBKT7. The bZIP domain and four conserved domains are indicated. (C) Transactivation analysis of CbABF1 in yeast. The constructs for expression of the GAL4 DNA-binding domain fused with CbABF1, CbABF1Δ1, and CbABF1Δ2 were co-transformed with empty <t>pGADT7</t> into yeast strain AH109, respectively. A 10-fold dilution series (10 -1 to 10 -3 ) of transformants was spotted on SD medium without Leu and Trp (–LW), or without Leu, Trp, and His (–LWH) plus 35 mM 3-amino-1,2,4-triazole (3-AT). The plates were incubated at 30°C for 4 days and subjected to the X-gal assay. Empty pGBKT7 with empty pGADT7 was used as the negative control.
Pgadt7 Rec, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgadt7 rec/product/TaKaRa
Average 86 stars, based on 1 article reviews
pgadt7 rec - by Bioz Stars, 2026-03
86/100 stars
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gal4 activation domain vector  (TaKaRa)
86
TaKaRa gal4 activation domain vector
CbABF1 subcellular localization and transactivation activity assays. (A) Localization of p35S:CbABF1-YFP in tobacco leaves, DAPI staining was used to indicate the nuclei. Bars = 15 μm. (B) A schematic diagram of the CbABF1 protein sequence and its truncated fragments that were fused to the GAL4 DNA-binding domain expressed from the yeast vector pGBKT7. The bZIP domain and four conserved domains are indicated. (C) Transactivation analysis of CbABF1 in yeast. The constructs for expression of the GAL4 DNA-binding domain fused with CbABF1, CbABF1Δ1, and CbABF1Δ2 were co-transformed with empty <t>pGADT7</t> into yeast strain AH109, respectively. A 10-fold dilution series (10 -1 to 10 -3 ) of transformants was spotted on SD medium without Leu and Trp (–LW), or without Leu, Trp, and His (–LWH) plus 35 mM 3-amino-1,2,4-triazole (3-AT). The plates were incubated at 30°C for 4 days and subjected to the X-gal assay. Empty pGBKT7 with empty pGADT7 was used as the negative control.
Gal4 Activation Domain Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gal4 activation domain vector/product/TaKaRa
Average 86 stars, based on 1 article reviews
gal4 activation domain vector - by Bioz Stars, 2026-03
86/100 stars
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matchmaker ad cloning vector  (Becton Dickinson)
90
Becton Dickinson matchmaker ad cloning vector
CbABF1 subcellular localization and transactivation activity assays. (A) Localization of p35S:CbABF1-YFP in tobacco leaves, DAPI staining was used to indicate the nuclei. Bars = 15 μm. (B) A schematic diagram of the CbABF1 protein sequence and its truncated fragments that were fused to the GAL4 DNA-binding domain expressed from the yeast vector pGBKT7. The bZIP domain and four conserved domains are indicated. (C) Transactivation analysis of CbABF1 in yeast. The constructs for expression of the GAL4 DNA-binding domain fused with CbABF1, CbABF1Δ1, and CbABF1Δ2 were co-transformed with empty <t>pGADT7</t> into yeast strain AH109, respectively. A 10-fold dilution series (10 -1 to 10 -3 ) of transformants was spotted on SD medium without Leu and Trp (–LW), or without Leu, Trp, and His (–LWH) plus 35 mM 3-amino-1,2,4-triazole (3-AT). The plates were incubated at 30°C for 4 days and subjected to the X-gal assay. Empty pGBKT7 with empty pGADT7 was used as the negative control.
Matchmaker Ad Cloning Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/matchmaker ad cloning vector/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
matchmaker ad cloning vector - by Bioz Stars, 2026-03
90/100 stars
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pgadt7-rec vector  (Promega)
90
Promega pgadt7-rec vector
Homodimeric and heterodimeric analysis of BplMYBs using a yeast two-hybrid assay (Y2H). ( A ) BplMYB46 was cloned into a <t>pGADT7-Rec</t> vector (AD-prey) and interacted separately with the eight MYB proteins, which were fused to the GAL4 DNA binding domain in the yeast pGBKT7 vector (BD-baits). Empty AD-prey and each BD-bait were used as negative controls; AD-BplMYB46-prey and empty BD-bait were used as negative controls; and AD-T-prey and BD-53-bait were used as positive controls. ( B ) BplMYB46-N (without its activation regions) was cloned into pGBKT7 vector (bait) and interacted with itself and with the other eight MYB proteins cloned into the pGADT7-Rec vector (preys). Empty BD-bait and each AD-prey were used as negative controls; BD-BplMYB46-N-bait and empty AD-prey were used as negative controls; BD-53-bait and AD-T-prey were used as positive controls. The yeast cells were grown on SD/-Trp/-Leu (double dropout (DDO)) and selective dropout media: SD/-Trp/-Leu/-His/-Ade/X-α-Gal (quadruple dropout (QDO)/X-α-Gal).
Pgadt7 Rec Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgadt7-rec vector/product/Promega
Average 90 stars, based on 1 article reviews
pgadt7-rec vector - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


CbABF1 subcellular localization and transactivation activity assays. (A) Localization of p35S:CbABF1-YFP in tobacco leaves, DAPI staining was used to indicate the nuclei. Bars = 15 μm. (B) A schematic diagram of the CbABF1 protein sequence and its truncated fragments that were fused to the GAL4 DNA-binding domain expressed from the yeast vector pGBKT7. The bZIP domain and four conserved domains are indicated. (C) Transactivation analysis of CbABF1 in yeast. The constructs for expression of the GAL4 DNA-binding domain fused with CbABF1, CbABF1Δ1, and CbABF1Δ2 were co-transformed with empty pGADT7 into yeast strain AH109, respectively. A 10-fold dilution series (10 -1 to 10 -3 ) of transformants was spotted on SD medium without Leu and Trp (–LW), or without Leu, Trp, and His (–LWH) plus 35 mM 3-amino-1,2,4-triazole (3-AT). The plates were incubated at 30°C for 4 days and subjected to the X-gal assay. Empty pGBKT7 with empty pGADT7 was used as the negative control.

Journal: Frontiers in Plant Science

Article Title: A Cryophyte Transcription Factor, CbABF1, Confers Freezing, and Drought Tolerance in Tobacco

doi: 10.3389/fpls.2019.00699

Figure Lengend Snippet: CbABF1 subcellular localization and transactivation activity assays. (A) Localization of p35S:CbABF1-YFP in tobacco leaves, DAPI staining was used to indicate the nuclei. Bars = 15 μm. (B) A schematic diagram of the CbABF1 protein sequence and its truncated fragments that were fused to the GAL4 DNA-binding domain expressed from the yeast vector pGBKT7. The bZIP domain and four conserved domains are indicated. (C) Transactivation analysis of CbABF1 in yeast. The constructs for expression of the GAL4 DNA-binding domain fused with CbABF1, CbABF1Δ1, and CbABF1Δ2 were co-transformed with empty pGADT7 into yeast strain AH109, respectively. A 10-fold dilution series (10 -1 to 10 -3 ) of transformants was spotted on SD medium without Leu and Trp (–LW), or without Leu, Trp, and His (–LWH) plus 35 mM 3-amino-1,2,4-triazole (3-AT). The plates were incubated at 30°C for 4 days and subjected to the X-gal assay. Empty pGBKT7 with empty pGADT7 was used as the negative control.

Article Snippet: The purified double-stranded cDNA was inserted into a Sma I-linearized pGADT7-Rec vector (3 μg) with T4 DNA ligase (New England Biolabs, Ipswich, MA, United States).

Techniques: Activity Assay, Staining, Sequencing, Binding Assay, Plasmid Preparation, Construct, Expressing, Transformation Assay, Incubation, Negative Control

Homodimeric and heterodimeric analysis of BplMYBs using a yeast two-hybrid assay (Y2H). ( A ) BplMYB46 was cloned into a pGADT7-Rec vector (AD-prey) and interacted separately with the eight MYB proteins, which were fused to the GAL4 DNA binding domain in the yeast pGBKT7 vector (BD-baits). Empty AD-prey and each BD-bait were used as negative controls; AD-BplMYB46-prey and empty BD-bait were used as negative controls; and AD-T-prey and BD-53-bait were used as positive controls. ( B ) BplMYB46-N (without its activation regions) was cloned into pGBKT7 vector (bait) and interacted with itself and with the other eight MYB proteins cloned into the pGADT7-Rec vector (preys). Empty BD-bait and each AD-prey were used as negative controls; BD-BplMYB46-N-bait and empty AD-prey were used as negative controls; BD-53-bait and AD-T-prey were used as positive controls. The yeast cells were grown on SD/-Trp/-Leu (double dropout (DDO)) and selective dropout media: SD/-Trp/-Leu/-His/-Ade/X-α-Gal (quadruple dropout (QDO)/X-α-Gal).

Journal: International Journal of Molecular Sciences

Article Title: BplMYB46 from Betula platyphylla Can Form Homodimers and Heterodimers and Is Involved in Salt and Osmotic Stresses

doi: 10.3390/ijms20051171

Figure Lengend Snippet: Homodimeric and heterodimeric analysis of BplMYBs using a yeast two-hybrid assay (Y2H). ( A ) BplMYB46 was cloned into a pGADT7-Rec vector (AD-prey) and interacted separately with the eight MYB proteins, which were fused to the GAL4 DNA binding domain in the yeast pGBKT7 vector (BD-baits). Empty AD-prey and each BD-bait were used as negative controls; AD-BplMYB46-prey and empty BD-bait were used as negative controls; and AD-T-prey and BD-53-bait were used as positive controls. ( B ) BplMYB46-N (without its activation regions) was cloned into pGBKT7 vector (bait) and interacted with itself and with the other eight MYB proteins cloned into the pGADT7-Rec vector (preys). Empty BD-bait and each AD-prey were used as negative controls; BD-BplMYB46-N-bait and empty AD-prey were used as negative controls; BD-53-bait and AD-T-prey were used as positive controls. The yeast cells were grown on SD/-Trp/-Leu (double dropout (DDO)) and selective dropout media: SD/-Trp/-Leu/-His/-Ade/X-α-Gal (quadruple dropout (QDO)/X-α-Gal).

Article Snippet: The open reading frame (ORF) of the BplMYB46 cDNA, without the terminal codon, was inserted into the pGADT7-Rec vector via its SmaI (Promega, Madison, WI, USA) site as the prey construct.

Techniques: Y2H Assay, Clone Assay, Plasmid Preparation, Binding Assay, Activation Assay